山羊MSTN基因多态性与主要经济性状的关联分析

doi:10.11843/j.issn.0366-6964.2015.09.005

Abstract

Abstract:

This experiment was designed to study the polymorphisms of Myostatin(MSTN) gene and its effects on body weight，body measurement and carcass traits in goats.The polymorphisms of 5′-regulatory region and exon 3 of MSTN gene in some goat populations were detected by PCR-RFLP and PCR-SSCP in the present study，respectively.The results showed that：The transition(C→T) at －662 bp in the 5′- regulatory region leaded to polymorphisms of TaqⅠ and there were CC，CT and TT genotypes in the 7 populations.The frequency of genotype CC in Boer goat was higher than that in the 3 indigenous goat populations，but the frequency of genotype TT in Boer goat was lower than that in the 3 indigenous goat populations.The frequency of allele C was higher than that of allele T in the Boer goats，while it was contrary in the 3 indigenous goat populations.Except for Boer goat populations，5 other populations were at Hardy-Weinberg nonequilibrium(P<0.01).Three genotypes NN，NM and MM were found in exon 3 of MSTN gene in 7 populations.The individuals with genotype CT-NN and CT-NM were not detected in the 7 populations.The individuals with genotype TT-NN and TT-NM were not detected in Boer goats，and the individuals with genotype TT-NM were also not found in F1.Furthermore，individuals with genotype TT-MM were also not observed in 3 indigenous goat populations.The correlations between the polymorphisms of MSTN gene 5′-regulatory region and weaning weight，meat weight and hindquarters meat weight were very significant(P<0.01).The average values of weaning weight and meat weight of individuals with genotype CC were significantly higher than that of individuals with genotype TT(P<0.01 in weaning weight，P<0.05 in meat weight) and the average values of hindquarters meat weight were not significantly different among different genotypes(P>0.05).The average values of weaning weight，meat weight and hindquarters meat weight in genotypes CC，CT and TT decreased in turn.At the same time，the significant correlations between the polymorphisms of MSTN gene 5′-regulatory region and liver weight was found(P<0.05).The average values of liver weight of individuals with genotype CT was significantly higher than that of individuals with genotype TT and the significant difference in liver weight among other genotypes were not observed(P>0.05).The significant correlations between the polymorphisms of exon 3 and meat weight，hindquarters meat weight and liver weight were observed(P<0.01).The genetic effects for genotypes were MM>NM>NN and the average values of meat weight，hindquarters meat weight and liver weight of individuals with genotype MM were significantly higher than that of individuals with genotype NN(P<0.01 in meat weight，P<0.05 in hindquarters meat weight and liver weight).The genetic interaction between exon 3 and 5′-regulatory region affected significantly weaning weight，hindquarters meat weight and liver weight(P<0.05).The average values of the 3 indexes of individuals with genotype CC-MM were the highest among the compound genotypes.Therefore，the different MSTN genotypes might be used as molecular genetic marker to select body weight，body measurement or carcass traits in goats.

CLC Number:

S827
S813.3

MIN Ling-jiang，FENG Yan-ni，LI Lan，LI Mei-yu. Associations of MSTN Gene’s Polymorphisms with Some Economic Traits in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1515-1524.

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Associations of MSTN Gene’s Polymorphisms with Some Economic Traits in Goats

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